Lipid peroxidation assay protocol summary:
– add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
– swap to wells of microplate
– analyze with microplate reader
For higher sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis.
Lipid Peroxidation (MDA + HNE) Assay Bundle
Lipid peroxidation is a extensively identified occasion of oxidative hurt in cell membranes, lipoproteins, and totally different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl ldl cholesterol can occur in a number of reactions. They’re usually triggered by i) free radical species similar to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated low cost of hydrogen peroxide or ii) non-radical species similar to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.
Malondialdehyde (MDA) and 4-hydroxyalkenals are important toxic byproducts of lipid peroxidation. So, the measurement of the portions of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a severe product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Furthermore, elevated ranges of HNE had been current in plasma and diverse organs beneath oxidative stress circumstances. The reality is, MDA is in numerous conditions most likely probably the most ample specific individual aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and loads of totally different biomolecules.
Bioquochem’s LPO assay gear measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). Due to this, this response offers a diindolylalkane (chromophore) with most absorbance inside the space of 580-620 nm.

In our assay an indol (Reagent A) reacts quickly with MDA and HNE in acidic medium, yielding a chromophore (C) with a extreme molar extinction coefficient at its maximal absorption wavelength of 586 nm.
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Product overview
Lipid Peroxidation (MDA) Assay Bundle (Colorimetric/Fluorometric) (ab118970) provides a helpful software program for delicate detection of malondialdehyde (MDA).
Inside the lipid peroxidation assay protocol, the MDA inside the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct might be merely quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/successfully colorimetrically and 0.1 nmol/successfully fluorometrically.
The MDA assay can be refered to as a TBARS assay.
Chinese language language protocol on the market. See protocols half beneath.
For an alternate MDA assay, with out the heating steps required inside the TBARS assay, try MDA assay ab233471.
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Notes
Lipid peroxidation refers again to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (usually in cell membranes), resulting in cell hurt. Quantification of lipid peroxidation is essential to judge oxidative stress. Lipid peroxidation varieties reactive aldehydes similar to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are typically used as markers of lipid peroxidation, and to assay for oxidative hurt / oxidative stress.
Related merchandise
Evaluation the oxidative stress marker and assay info, or the whole metabolism assay info to check additional assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and as well as how one can assay metabolic function in dwell cells using your plate reader.
Moreover see the favored 4-HNE Assay Bundle ab238538 instead marker of lipid peroxidation and oxidative stress.
How totally different researchers have used Lipid Peroxidation Assay Bundle ab118970The MDA/TBARs assay gear has been utilized in publications in a variety of sample types, along with:
– Human: serum1, hippocampal principal cell extracts2, A375 cultured cell lysates3, plasma and platelet samples4
– Mouse: neuronal cell lysates5, coronary coronary heart tissue extract6, plasma7, cell extracts8
– Rat: hippocampal tissue extracts9, cardiomyocyte extracts of cultured cells10, lung lysates11
– Pig: serum12References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018, 4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019
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Platform
Microplate reader
Lipid Peroxidation Colorimetric Assay Kit |
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K454-100 | Biovision | each | EUR 652.8 |
Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit |
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K739-100 | Biovision | each | EUR 698.4 |
Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit |
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K2167-100 | ApexBio | 100 assays | EUR 703.2 |
Cell Meterâ„¢ Fluorimetric Cellular Lipid Peroxidation Assay Kit |
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22906-200Tests | AAT Bioquest | 200 Tests | EUR 334 |
Description: Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS). |
Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit |
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22906 | AAT Bioquest | 200 Tests | EUR 334 |
Cell Meterâ„¢ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit |
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15991-200tests | AAT Bioquest | 200 tests | EUR 446 |
Description: Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol. |
Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit |
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15991 | AAT Bioquest | 200 tests | EUR 446 |
Myeloperoxidase (MPO) Peroxidation Activity Assay Kit |
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K747-100 | Biovision | each | EUR 392.4 |
OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric) |
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STA-805 | Cell Biolabs | 192 assays | EUR 692.4 |
Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample. |
Dog Lipid peroxide ELISA kit |
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E08L0263-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Lipid peroxide ELISA kit |
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E08L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Lipid peroxide ELISA kit |
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E08L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Lipid peroxide ELISA kit |
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E02L0263-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Lipid peroxide ELISA kit |
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E02L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Lipid peroxide ELISA kit |
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E02L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Lipid peroxide ELISA kit |
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E07L0263-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Porcine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Lipid peroxide ELISA kit |
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E07L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Porcine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Lipid peroxide ELISA kit |
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E07L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Porcine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Lipid peroxide ELISA kit |
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E06L0263-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Lipid peroxide ELISA kit |
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E06L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Lipid peroxide ELISA kit |
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E06L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Lipid peroxide ELISA kit |
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E03L0263-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Lipid peroxide ELISA kit |
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E03L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Lipid peroxide ELISA kit |
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E03L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Lipid peroxide ELISA kit |
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E01L0263-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Human Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Lipid peroxide ELISA kit |
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E01L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Human Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Lipid peroxide ELISA kit |
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E01L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Human Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Lipid peroxide ELISA kit |
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E04L0263-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Lipid peroxide ELISA kit |
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E04L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Lipid peroxide ELISA kit |
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E04L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |