Accenx Technologies, Inc.

Principle & Course of
The ChromaFlash Chromatin Extraction Bundle accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Background Knowledge

Chromatin immunoprecipitaton (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space under quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with explicit antibodies straight in the direction of quite a few transcriptional parts is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Bundle is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this package deal will be utilized in numerous chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Bundle. The isolated chromatin can even be utilized in several chromatin-related capabilities equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

• Terribly fast course of: your whole course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
• Useful and versatile: the package deal is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
• Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 10⁵ to 5 x 10⁶ cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 10⁶ cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 10⁶  cells or per 50 mg tissues.

Background Knowledge
Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space under quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with explicit antibodies straight in the direction of quite a few transcriptional parts is broadly in demand.

EpiQuik Chromatin Accessibility Assay Kit
P-1047	EpiGentek	EUR 910.86 EUR 611.60	48 Assays 48 Reactions

miRNA Extraction Kit
K1456-50	Biovision	50 Preps	EUR 661.2

gPURE EXTRACTION KIT
IB47430	IBI Scientific	3ML	EUR 30.42
Description: (FOR CULTURED CELLS)

gPURE EXTRACTION KIT
IB47431	IBI Scientific	100ML	EUR 196.06
Description: (FOR CULTURED CELLS)

gPURE EXTRACTION KIT
IB47432	IBI Scientific	1000ML	EUR 1358.49
Description: (FOR CULTURED CELLS)

ExoDNA? Extraction Kit
K1242-25	Biovision	each	EUR 705.6

ExoDNA? Extraction Kit
K1242-50	Biovision	each	EUR 1057.2

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
P-2002	EpiGentek	EUR 1084.86 EUR 312.40 EUR 500.50 EUR 738.10	96 Reactions 24 Reactions 48 Reactions 96 Reactions

Up RNA Extraction Kit
20-abx098087	Abbexa	EUR 309.60 EUR 126.00	100 ml 3 ml

Chromatin protein Cren7 Antibody (HRP)
20-abx109204	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Chromatin protein Cren7 Antibody (FITC)
20-abx107787	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Chromatin Modifying Protein 2A Protein
20-abx261989	Abbexa	EUR 5388.00 EUR 393.60 EUR 276.00	1 mg 20 ug 5 ug

Chromatin Modifying Protein 2B Protein
20-abx262025	Abbexa	EUR 5388.00 EUR 393.60 EUR 276.00	1 mg 20 ug 5 ug

Chromatin Modifying Protein 4A Protein
20-abx263203	Abbexa	EUR 276.00 EUR 1779.60 EUR 393.60	1 µg 50 ug 5 ug

Chromatin Modifying Protein 1A Protein
20-abx263326	Abbexa	EUR 1930.80 EUR 393.60 EUR 276.00	100 ug 10 ug 2 µg

Chromatin Protein Cren7 (creN7) Antibody
20-abx109437	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

ATRX, Chromatin Remodeler (ATRX) Antibody
20-abx325123	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

ATRX, Chromatin Remodeler (ATRX) Antibody
abx331300-100ul	Abbexa	100 ul	EUR 510

Quick Gel Extraction Kit
20-abx098085	Abbexa	EUR 343.20 EUR 243.60	200 rxns 50 rxns

Plant RNA extraction Kit
20-abx098088	Abbexa	EUR 309.60 EUR 126.00	100 ml 3 ml

VIRAL DNA EXTRACTION KIT
IB47400	IBI Scientific	4 PREPS	EUR 30.11

VIRAL DNA EXTRACTION KIT
IB47401	IBI Scientific	50 PREPS	EUR 238.33

VIRAL DNA EXTRACTION KIT
IB47402	IBI Scientific	100 PREPS	EUR 428.75

VIRAL DNA EXTRACTION KIT
IB47403	IBI Scientific	300 PREPS	EUR 1151.98

Chromatin protein Cren7 Antibody (Biotin)
20-abx106375	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Gel Extraction Kit (100 preps)
9K-006-0001	Bio Basic	100preps	EUR 283.42

Gel Extraction Kit (200 preps)
9K-006-0002	Bio Basic	200preps, 200prep	EUR 418.09

FFPE Protein Extraction Kit
BSP058	Bio Basic	50Preps	EUR 122.64

Exosomal RNA Extraction Kit
P200R,-part-of-P200	101Bio	-	Ask for price

Exosomal DNA Extraction Kit
P230-25	101Bio	-	Ask for price

Exosomal DNA Extraction Kit
P230-50	101Bio	-	Ask for price

Plant Tissue Extraction Kit
K296-50	Biovision	each	EUR 548.4

Exosomal RNA Extraction Kit
P200R	101Bio	20 rxn	EUR 389

Human Chromatin Regulation Primer Library
HCHR-I	Real Time Primers	1 set	EUR 774

EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit
P-2003	EpiGentek	EUR 1084.86 EUR 312.40 EUR 500.50 EUR 738.10	96 Reactions 24 Reactions 48 Reactions 96 Reactions

Total Protein Extraction Kit
BSP003	Bio Basic	50Preps	EUR 130.99

Plant Protein Extraction Kit
BSP004	Bio Basic	20Preps	EUR 133.08

Total Protein Extraction Kit
K3011010	Biochain	1 kit	EUR 367

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