human igg elisa / human igg4 isotype control / pdk4 antibody / primary cell transfection

A Modified Simple Method for Induction of Myocardial Infarction in Mice

- June 30, 2022 - Ariana
  • Myocardial infarction (MI) represents one of the leading causes of death. MI models are widely used for investigating the pathomechanisms of post-MI remodeling and evaluation of novel therapeutics. Different methods (e.g., isoproterenol treatment, cryoinjury, coronary artery ligation, etc.) have been used to induce MI. Compared with isoproterenol treatment and cryoinjury, coronary artery ligation may better reflect the ischemic response and chronic remodeling after MI. However, traditional methods for coronary ligation in mice are technically challenging and require commercially available apparatus. The current study describes a simple and efficient process for induction of MI in mice with readily available materials.
  • The mouse chest skin was cut open under stable anesthesia with a simplified anesthesia device made of centrifuge tubes. The heart was immediately externalized through the intercostal space after blunt separation of the pectoralis major and pectoralis minor. The left anterior descending branch (LAD) was ligated with a 6-0 suture 3 mm from its origin. Following LAD ligation, staining with 2,3,5-Triphenyltetrazolium chloride (TTC) indicated successful induction of MI and temporal changes of post-MI scar size. Meanwhile, survival analysis results showed overt mortality within 7 days after MI, mainly due to cardiac rupture. Moreover, post-MI echocardiographic assessment demonstrated successful induction of contractile dysfunction and ventricular remodeling. Once mastered, an Corning Centrifuge Tubes MI model can be established in mice within 2-3 min with readily available materials.

Extending the working properties of liquid platelet-rich fibrin using chemically modified PET tubes and the Bio-Cool device

Centrifuge Tube
Centrifuge Tube
  1. Platelet-rich fibrin (PRF) has been utilized in regenerative medicine as a concentration of autologous platelets and growth factors that stimulates tissue regeneration. More recently, liquid-PRF (also called injectable-PRF; i-PRF) has been brought to market utilizing PET plastic tubes. Due to new advances made in tube technology, the first aim of the present study was to investigate the liquid consistency of liquid-PRF utilizing both standard and chemically modified PET plastic tubes. Furthermore, it is well known that the conversion of PRF into a fibrin matrix is derived from the temperature-controlled enzymatic process that converts liquid fibrinogen and thrombin to solid fibrin. This study also investigated for the first time the use of a cooling device (Bio-Cool) to extend the liquid working properties of liquid-PRF.
  2. In total, 30 participants enrolled in this study. From each patient, four tubes of liquid-PRF were drawn, two standard white Vacuette tubes and two blue chemically modified hydrophobic tubes. Following centrifugation at 700 RCF-max for 8 min in a Bio-PRF horizontal centrifuge, one white and one blue tube were kept upright at room temperature, while the other white and blue tube were placed within the cooling device. Thereafter, the liquid-PRF layers were monitored over time until clotting occurred. Patient gender, age, and altitude above sea level (+ 5000 ft) were recorded and compared for clotting times.
  3. The findings from the present study demonstrated that the chemically modified PET tubes performed 37% better than the control tubes (extended the working properties of liquid-PRF by over 20 min). Most surprisingly, tubes kept in the cooling device demonstrated an average of 90 min greater working time (270% improvement). While patients living at altitude did significantly improve the clotting ability of liquid-PRF, no differences were observed when comparing male vs female or younger vs older patients in liquid-PRF clotting times.
  4. Cooling of blood following centrifugation represented a 270% improvement in working properties of liquid-PRF. Optimization of liquid-PRF tubes utilizing chemically modified hydrophobic PET tubes also delayed the clotting process by 37%. Patient gender and age had little relevance on liquid-PRF.
  5. The present findings demonstrate for the first time that cooling of liquid-PRF is able to extend the working properties of liquid-PRF by over 90 min. Thus for clinicians performing longer clinical procedures, the cooling of blood may represent a viable strategy to improve the working time of liquid-PRF in clinical practice. Bio-PRF; Cooling device; PRF; Platelet-rich fibrin; Wound healing.

Fixation and Immunostaining of Endogenous Proteins or Post-translational Modificationsin Caenorhabditis elegant.

  • Although the advent of genetically-encoded fluorescent markers, such as the green fluorescent protein (GFP; Chalfie et al., 1994 ), has enabled convenient visualization of gene expression in vivo, this method is generally not effective for detecting post-translational modifications because they are not translated from DNA sequences.
  • Genetically-encoded, fluorescently-tagged transgene products can also be misleading for observing expression patterns because transgenes may lack endogenous regulatory DNA elements needed for precise regulation of expression that could result in over or under expression. Fluorescently-tagged proteins created by CRISPR genome editing are less prone to defective expression patterns because the loci retain endogenous DNA elements that regulate their transcription (Nance and Frøkjær-Jensen, 2019).
  • However, even CRISPR alleles encoding heritable fluorescently-tagged protein markers can result in defects in function or localization of the gene product if the fluorescent tag obstructs or otherwise interferes with important protein interaction domains or affects the protein structure. Indirect immunofluorescence is a method for detecting endogenous gene expression or post-translational modifications without the need for transgenesis or genome editing.
  • Here, we present a reliable protocol in which C. elegans nematodes are fixed, preserved, and permeabilized for staining with a primary antibody to bind proteins or post-translational modifications, which are then labeled with a secondary antibody conjugated to a fluorescent dye.
  • Use of this method may be limited by the availability of (or ability to generate) a primary antibody that binds the epitope of interest in fixed animals. Thousands of animals are simultaneously subjected to a series of chemical treatments and washes in a single centrifuge tube, allowing large numbers of identically-treated stained animals to be examined.
  • We have successfully used this protocol (O’ Hagan et al., 2011 and 2017; Power et al., 2020 ) to preserve and detect post-translational modifications of tubulin in C. elegans ciliated sensory neurons and to detect non-modified endogenous protein (Topalidou and Chalfie, 2011).

Transfer some Hydrogel Containing 5-Fluorouracil and Etodolac Combination for Synergistic Oral Cancer Treatment

  • Oral cancer is one of the most common malignancies with an increased rate of incidence. 5-Fluorouracil (5FU) is an effective chemotherapeutic indicated for oral cancer treatment. Etodolac (Et), a cyclooxygenase-2 inhibitor, can be used as an adjuvant agent to sensitize cancer cells to chemotherapy. The aim of this work was to prepare and characterize 5FU and Et dual drug-loaded transfersomes to treat oral cancer.
  • Transfersomes were prepared by thin-film hydration method and characterized for the average particle size and zeta-potential using dynamic light scattering and scanning electron microscopy techniques.
  • The prepared transfersomes were further characterized for their drug loading, entrapment efficiencies using amicon centrifuge tubes and drug release behavior using cellulose membrane. The synergistic activity of dual drug-loaded transfersomes was studied in FaDu oral cancer cells. Results showed that the average particle size, polydispersity index, and zeta potential were 91±6.4 nm, 0.28±0.03, and (-)46.9±9.5 mV, respectively, for 5FU- and Et (1:1)-loaded transfersomes.

Centrifuge Tubes, 15 ml ,PP,Sterile,Plug-Seal Cap, in Bag
LC8003-500 GenDepot 500/CS

Centrifuge Tubes, 50 ml ,PP,Sterile,Plug-Seal Cap, in Bag
LC8005-500 GenDepot 500/CS

Centrifuge Tubes, 15 ml , Black ,PP,Sterile
LC8007-050 GenDepot 50/Bag

Centrifuge Tubes, 15 ml , Black ,PP,Sterile
LC8007-500 GenDepot 500/CS

Centrifuge Tubes, 15 ml , Amber ,PP,Sterile
LC8015-500 GenDepot 500/CS

Centrifuge Tubes, 50 ml , Black ,PP,Sterile
LC8008-025 GenDepot 25/Bag

Centrifuge Tubes, 50 ml , Black ,PP,Sterile
LC8008-500 GenDepot 500/CS

Centrifuge Tubes, 50 ml , Amber ,PP,Sterile
LC8050-500 GenDepot 500/CS

Centrifuge Tubes, 50 ml ,PP,Sterile,Plug-Seal Cap,Self Standing, in Bag
LC8006-500 GenDepot 500/CS

15 mL Centrifuge Tube, Tubes and Caps Packed Separately, Non-Sterile, 50/pk, 500
601151 NEST Biotechnology 500 pcs/cs

Block, 15 x1.5ml centrifuge tubes
BCM1405-B15 Bio Basic 1 pcs, 1 UNIT

Block, 15 x1.5ml centrifuge tubes
BSH100-15 Benchmark Scientific 1 PC

50ml Conical Centrifuge Tubes, Green Cap, 25/Bag
CT788-G Bio Basic 1PK, 25UNIT

50ml Conical Centrifuge Tubes, Yellow Cap, 25/Bag
CT788-Y Bio Basic 1PK, 25UNIT

Stand For Centrifuge Tubes For 1.5 ml,
PW102-2NO EWC Diagnostics 1 unit

16 mm Test Tube Foam Rack (for 15 mL centrifuge tubes) - EACH
MIX1098 Scientific Laboratory Supplies EACH

Centrifuge Tubes, 15 ml ,PP,Sterile,Plug-Seal Cap, in Rack
LC8002-500 GenDepot 500/CS

29 mm Test Tube Foam Rack (for 50 mL centrifuge tubes) - EACH
MIX1118 Scientific Laboratory Supplies EACH

Centrifuge Tubes, 50 ml ,PP,Sterile,Plug-Seal Cap, in Rack
LC8004-500 GenDepot 500/CS

Corning LSE Single Block 5 x 50 mL Centrifuge Tubes - EACH
480129 Scientific Laboratory Supplies EACH

4-Ways Microtube and Centrifuge Tubes Racks, Red
R023-R Bio Basic 1 UNIT

15 mL Centrifuge Tube Rack, 1/pk, 50/cs
610001 NEST Biotechnology 50 pcs/cs

4-Ways Microtube and Centrifuge Tubes Racks, Blue
R023-B Bio Basic 1 UNIT

Greiner centrifuge tubes - PK300
T2193-300EA Scientific Laboratory Supplies PK300

Greiner centrifuge tubes - PK1000
T1943-1000EA Scientific Laboratory Supplies PK1000

4-Ways Microtube and Centrifuge Tubes Racks, Yellow
R023-Y Bio Basic 1 UNIT

Block, 15 x 1.5ml or 2.0ml centrifuge tubes (conical)
BCM1405-B1520 Bio Basic 1 pcs, 1 UNIT

Block, 15 x 1.5ml or 2.0ml centrifuge tubes (conical)
BSH100-1520 Benchmark Scientific 1 PC

4-Ways Microtube and Centrifuge Tubes Racks, Natural
R023-N Bio Basic 1 UNIT

Block for 5ml centrifuge tubes
H5000-5MT Benchmark Scientific 1 PC

Centrifuge Tubes, Autoclavable BLUE
CG264-1X100NO EWC Diagnostics 1 unit

Centrifuge Tubes, Autoclavable Round
CG276-1X100NO EWC Diagnostics 1 unit

Centrifuge Tubes, Autoclavable Round
CG277-1X100NO EWC Diagnostics 1 unit

Centrifuge Tubes, Autoclavable Round
CG278-1X100NO EWC Diagnostics 1 unit

Centrifuge Tubes, Autoclavable Round
CG279-1X100NO EWC Diagnostics 1 unit

15mL Sterile Centrifuge Tubes PP
ARG1818 Scientific Laboratory Supplies PK500

50mL Sterile Centrifuge Tubes PP
ARG1824 Scientific Laboratory Supplies PK500

Block, 20x10mm test tubes or 20x2.0ml centrifuge tubes
BCM1412-BSW10 Bio Basic 1 pcs, 1 UNIT

Block, 20x10mm test tubes or 20x2.0ml centrifuge tubes
BSW10 Benchmark Scientific 1 PC

Centrifuge Tubes, Autoclavable Conical
CG274-100X1NO EWC Diagnostics 1 unit

CapLock 0.5mL Centrifuge Tubes - PK500
ARG1782 Scientific Laboratory Supplies PK500

Oakridge Centrifuge Tubes 30ml - PK10
31390030 Scientific Laboratory Supplies PK10

CapLock 1.5mL Centrifuge Tubes - PK1000
ARG1808 Scientific Laboratory Supplies PK1000

Block, 24 x 0.5ml centrifuge tubes
BCM1405-B05 Bio Basic 1 pcs, 1 UNIT

Block, 24 x 0.5ml centrifuge tubes
BCM1412-BSW05 Bio Basic 1 pcs, 1 UNIT

Block, 24 x 0.5ml centrifuge tubes
BSW05 Benchmark Scientific 1 PC

Block, 24 x 0.5ml centrifuge tubes
BSH100-05 Benchmark Scientific 1 PC

Centrifuge Tubes S 2420 30ml Qfit - PK10
QBC24C24R0 Scientific Laboratory Supplies PK10

15 mL Centrifuge Tube, Bulk, Sterile, 50/pk, 500/cs
601052 NEST Biotechnology 500 pcs/cs

Block, 4 x 15ml centrifuge tubes
BCM1405-B150 Bio Basic 1 pcs, 1 UNIT

Block, 2 x 50ml centrifuge tubes
BCM1405-B500 Bio Basic 1 pcs, 1 UNIT

Block, 12 x 15ml centrifuge tubes
BCM1412-BSW15 Bio Basic 1 pcs, 1 UNIT

Block, 5 x 50ml centrifuge tubes
BCM1412-BSW50 Bio Basic 1 pcs, 1 UNIT

Block, 4 x 15ml centrifuge tubes
BSH100-150 Benchmark Scientific 1 PC

Block, 2 x 50ml centrifuge tubes
BSH100-500 Benchmark Scientific 1 PC

Block, 12 x 15ml centrifuge tubes
BSW15 Benchmark Scientific 1 PC

Block, 5 x 50ml centrifuge tubes
BSW50 Benchmark Scientific 1 PC

Centrifuge Tubes, with ring (leak proof)
PW1207-1X100NO EWC Diagnostics 1 unit

Centrifuge Tubes, Autoclavable RED Cap,
CG272-1X100NO EWC Diagnostics 1 unit

Centrifuge Tubes, Autoclavable With RED
CG271-1X100NO EWC Diagnostics 1 unit

Block for 5mL centrifuge tubes - EACH
MIX7218 Scientific Laboratory Supplies EACH

Block, 24 x1.5ml centrifuge tubes (conical)
BCM1412-BSW1500 Bio Basic 1 pcs, 1 UNIT

Block, 24 x1.5ml centrifuge tubes (conical)
BSW1500 Benchmark Scientific 1 PC

15mL Sterile Centrifuge Tubes PP - PK500
ARG1814 Scientific Laboratory Supplies PK500

50mL Sterile Centrifuge Tubes PP - PK500
ARG1820 Scientific Laboratory Supplies PK500

50 mL Centrifuge Tube Rack, 1/pk, 50/cs
610101 NEST Biotechnology 50 pcs/cs

50ml Plug-Seal Centrifuge Tubes with Rack, 25 tubes/rack
10-0501 IHC World -

Falcon Conical 50ml PP Centrifuge Tubes Bagged - PK500
352070 Scientific Laboratory Supplies PK500

Falcon Conical 15ml PP Centrifuge Tubes Bagged - PK500
352096 Scientific Laboratory Supplies PK500

50ml Conical Centrifuge Tubes, Polypropylene
21-106 Genesee Scientific 20 Racks of 25 Tubes, 500/Unit

50ml Conical Centrifuge Tubes, Polypropylene
21-108 Genesee Scientific 20 Bags of 25 Tubes, 500/Unit

15ml Conical Centrifuge Tubes, Polypropylene
28-101 Genesee Scientific 20 Racks of 25 Tubes, 500/Unit

15ml Conical Centrifuge Tubes, Polypropylene
28-103 Genesee Scientific 20 Bags of 25 Tubes, 500/Unit

50ml Conical Centrifuge Tubes, Polypropylene
28-106 Genesee Scientific 20 Racks of 25 Tubes, 500/Unit

50ml Conical Centrifuge Tubes, Polypropylene
28-108 Genesee Scientific 20 Bags of 25 Tubes, 500/Unit

Centrifuge 5804 + Rotor S-4-72 + adapters 15/50ml tubes - EACH
E5804000565 Scientific Laboratory Supplies EACH

Eppendorf Adapter for 0.5 mL micro- centrifuge tubes + 0.6 ml Microtainer set of 6 - PK6
E5425716001 Scientific Laboratory Supplies PK6

Kimas high strength centrifuge tubes - PK6
Z514861-6EA Scientific Laboratory Supplies PK6

Centrifuge 5804R + Rotor S-4-72 + adapters 15/50ml tubes - EACH
E5805000665 Scientific Laboratory Supplies EACH

Centrifuge 5810 + Rotor S-4-104 + adapters 15/50ml tubes - EACH
E5810000560 Scientific Laboratory Supplies EACH

Tubes Centrifuge 15ml Conical - PK500
CEN9202 Scientific Laboratory Supplies PK500

Centrifuge 5810R + Rotor S-4-104 + adapters 15/50ml tubes - EACH
E5811000660 Scientific Laboratory Supplies EACH

SINGLE BLOCK, 12 X 15ML CENTRIFUGE TUBES
480124 CORNING 1/pk

Block, 24 x 1.5ml or 2.0ml centrifuge tubes
BCM1412-BSW1520 Bio Basic 1 pcs, 1 UNIT

Block, 24 x 1.5ml or 2.0ml centrifuge tubes
BSW1520 Benchmark Scientific 1 PC

Block, 15 x 1.5ml Centrifuge Tubes for Dry Bath with Heated Lid
BCM1405-B15HL Bio Basic 1 pcs, 1 UNIT

Block, 15 x 1.5ml Centrifuge Tubes for Dry Bath with Heated Lid
BSH100-15HL Benchmark Scientific 1 PC

5 mL Centrifuge Tubes, Clear, Round, Lock Cap, Sterile, 200/pk, 2000/cs
603111 NEST Biotechnology 2000 pcs/cs

15 mL Centrifuge Tube, Rack(PS Rack), Sterile, 50/pk, 500/cs
601051 NEST Biotechnology 500 pcs/cs

250mL centrifuge tube PP (59.8 X 160mm), with screw-cap, 14 bags of 5 tubes
C1250 MTC Bio 70

CENTRIFUGE TUBE 50 ML 30 X 115 MM - PK500
T2318-500EA Scientific Laboratory Supplies PK500

250 mL PP Centrifuge Tubes with Plug Seal Cap, Sterile, 6/pk, 102/cs
622001 NEST Biotechnology 102 pcs/cs

500 mL PP Centrifuge Tubes with Plug Seal Cap, Sterile, 6/pk, 36/cs
623001 NEST Biotechnology 36 pcs/cs

Falcon Conical 225ml PP Centrifuge Tubes - PK48
352075 Scientific Laboratory Supplies PK48

15mL and 50ml Universal Rack for Conical Bottom Centrifuge Tubes - PK20
4366 Scientific Laboratory Supplies PK20

Falcon Conical 15ml Ps Centrifuge Tubes - PK500
352099 Scientific Laboratory Supplies PK500

5-Well Centrifuge Tube Box (1.5ml To 2ml Tubes)
R005-G Bio Basic 1 UNIT

Block 15 x 1.5mL Centrifuge Tubes for Dry Bath with Heated Lid - EACH
BLO1254 Scientific Laboratory Supplies EACH

Block, 6 x 5ml centrifuge tubes (17mm diameter)
BCM1405-B5MT Bio Basic 1 pcs, 1 UNIT

Block, 12 x 5.0ml centrifuge tubes (17mm diameter)
BCM1412-BSW5MT Bio Basic 1 pcs, 1 UNIT

Block, 6 x 5ml centrifuge tubes (17mm diameter)
BSH100-5MT Benchmark Scientific 1 PC

Block, 12 x 5.0ml centrifuge tubes (17mm diameter)
BSW5MT Benchmark Scientific 1 PC

15ml Centrifuge Tube, Screw Cap, Natural, 100/Bag
BT888-N Bio Basic 1PK, 100UNIT

Centrifuge 5804 + Rotor S-4-72 + adapters 15/50ml tubes- IVD Only - EACH
E5804000560 Scientific Laboratory Supplies EACH

Block, 8x5ml centrifuge tubes, for MultiTherm Touch
H5100-5MT Benchmark Scientific 1 each

5 mL Centrifuge Tubes, Clear, Round, Lock Cap, Non-Sterile, 200/pk, 2000/cs
603011 NEST Biotechnology 2000 pcs/cs

Centrifuge Tube, 5ml, 200 per. pk. (4 bags of 50)
C1005-T5-Clear Benchmark Scientific 1 each

Centrifuge Tube, 5ml, 200 per. pk. (4 bags of 50)
C1005-T5_Clear Benchmark Scientific each

Centrifuge 5804R + Rotor S-4-72 + adapters 15/50ml tubes- IVD Only - EACH
E5805000661 Scientific Laboratory Supplies EACH

Centrifuge 5810 + Rotor S-4-104 + adapters 15/50ml tubes- IVD Only - EACH
E5810000564 Scientific Laboratory Supplies EACH

Centrifuge 5810R + Rotor S-4-104 + adapters 15/50ml tubes- IVD Only - EACH
E5811000665 Scientific Laboratory Supplies EACH

250 mL PP Centrifuge Tubes with Plug Seal Cap, Racked, Sterile, 6/pk, 24/cs
622002 NEST Biotechnology 24 pcs/cs

500 mL PP Centrifuge Tubes with Plug Seal Cap, Racked, Sterile, 6/pk, 24/cs
623002 NEST Biotechnology 24 pcs/cs

50mL Rack for Conical Bottom Centrifuge Tubes - PK20
4365 Scientific Laboratory Supplies PK20

15mL Rack for Conical Bottom Centrifuge Tubes - PK20
431355 Scientific Laboratory Supplies PK20

50ml Skirted Centrifuge Tube, Green Cap, 25/Bag
TK943-G Bio Basic 1PK, 25UNIT

Cool rack 9 wells for 15ml centrifuge tubes - EACH
HOM2740 Scientific Laboratory Supplies EACH

Cool rack 4 wells for 50ml centrifuge tubes - EACH
HOM2742 Scientific Laboratory Supplies EACH

High Speed Centrifuge, Adapt for 1.5/2.0 mL Microcentrifuge Tubes, 500-1200 RPM
102101 NEST Biotechnology 1 pcs/cs

Falcon Conical 175ml PP Centrifuge Tubes Racked - PK48
352076 Scientific Laboratory Supplies PK48

50ml Skirted Centrifuge Tube, Yellow Cap, 25/Bag
TK943-Y Bio Basic 1PK, 25UNIT

15ml Centrifuge Tube, Screw Cap, Red, Sterile, 100/Bag
BT888-SR Bio Basic 1PK, 100UNIT

Falcon Conical 15ml PP Centrifuge Tubes Racked - PK500
352097 Scientific Laboratory Supplies PK500

Falcon Conical 50ml PP Centrifuge Tubes Racked - PK500
352098 Scientific Laboratory Supplies PK500

Kimax Reuseable Glass Centrifuge tubes oil 203mm - PK12
TUB2716 Scientific Laboratory Supplies PK12

Block 6 x 5mL centrifuge tubes (17mm diameter) - EACH
BLO1256 Scientific Laboratory Supplies EACH

Block 12 x 5.0mL centrifuge tubes (17mm diameter) - EACH
BLO1276 Scientific Laboratory Supplies EACH

15ml Centrifuge Tube, Screw Cap, Blue, Sterile, 100/Bag
BT888-SB Bio Basic 1PK, 100UNIT

15ml Centrifuge Tube, Screw Cap, Yellow, Sterile, 100/Bag
BT888-SY Bio Basic 1PK, 100UNIT

50 mL Centrifuge Tube, Bulk, Sterile, 25/pk, 500/cs
602052 NEST Biotechnology 500 pcs/cs

Falcon Conical 15ml Ps Multipack Centrifuge Tubes - PK500
352095 Scientific Laboratory Supplies PK500

15 mL Centrifuge Tube, Amber Color, Rack(PS Rack), Sterile, 50/pk, 500/cs
601201 NEST Biotechnology 500 pcs/cs

CoolRack XT 5ml holds 12 x 5ml centrifuge tubes - EACH
432060 Scientific Laboratory Supplies EACH

15ml Centrifuge Tube, Screw Cap, Natural, Sterile, 100/Bag
BT888-SN Bio Basic 1PK, 100UNIT

15mL Flip Top Centrifuge Tubes Sterile DNase Free - PK500
ARG1154 Scientific Laboratory Supplies PK500

50mL Flip Top Centrifuge Tubes Sterile DNase Free - PK500
ARG1156 Scientific Laboratory Supplies PK500

50ml High Speed Centrifuge Tube, Natural cap, 25/Bag
CT790-N Bio Basic 1PK, 25UNIT

Snap Cap Polyethylene For Centrifuge Tubes Size 2 - PK500
TUB2728 Scientific Laboratory Supplies PK500

Benchmark Centrifuge Tube Red 5mL (4 bags of 50) - PK200
CEN2048 Scientific Laboratory Supplies PK200

Benchmark Centrifuge Tube Blue 5mL (4 bags of 50) - PK200
CEN2046 Scientific Laboratory Supplies PK200

50ml Skirted Centrifuge Tube, Green Cap, Sterile, 25/Bag
TK943-GS Bio Basic 1PK, 25UNIT

Benchmark Centrifuge Tube Clear 5mL (4 bags of 50) - PK200
CEN2045 Scientific Laboratory Supplies PK200

Benchmark Centrifuge Tube Green 5mL (4 bags of 50) - PK200
CEN2047 Scientific Laboratory Supplies PK200

Benchmark Centrifuge Tube Black 5mL (4 bags of 50) - PK200
CEN2051 Scientific Laboratory Supplies PK200

50ml Skirted Centrifuge Tube, Yellow Cap, Sterile, 25/Bag
TK943-YS Bio Basic 1PK, 25UNIT

Benchmark Centrifuge Tube Yellow 5mL (4 bags of 50) - PK200
CEN2049 Scientific Laboratory Supplies PK200

50 mL Centrifuge Tube, Rack (PS Rack), Sterile, 25/pk, 500/cs
602051 NEST Biotechnology 500 pcs/cs

Corning 15mL PP Centrifuge Tubes Rack Packed with CentriStar Cap Sterile 50/Rack 500/Case - PK500
430790 Scientific Laboratory Supplies PK500

50ml High Speed Centrifuge Tube, Natural cap, Sterile, 25/Bag
CT790-SN Bio Basic 1PK, 25UNIT

15ml centrifuge tubes, rack pack, sterile, flat top caps, graduation
SGB011-R SEEN GROUP LIMITED 50pcs/rack, 500pcs/carton

50ml centrifuge tubes, rack pack, sterile, flat top caps, graduation
SGB012-R SEEN GROUP LIMITED 25pcs/rack, 500pcs/carton

DB 2.1 Single block for 12 x 15ml conical centrifuge tubes - EACH
BLO6032 Scientific Laboratory Supplies EACH

Kimax Centrifuge tubes graduated beaded top white scale 10ml - PK12
TUB2708 Scientific Laboratory Supplies PK12

Kimax Reuseable Glass Centrifuge tubes plain high strength 15ml - PK6
TUB2718 Scientific Laboratory Supplies PK6

Centrifuge Tube, Conical, 500mL, PP, sterile, with screw-cap, 8 per bag, 5 bags per case, 40/cs
C1500 MTC Bio 40

Holder for up to eight 15ml centrifuge tubes, for Bench-Top Shaker
099ASR815 Glascol each

Holder for up to eight 50ml centrifuge tubes, for Bench-Top Shaker
099ASR850 Glascol each

50ml Conical Centrifuge Tubes, Green Cap, Sterile, 25unit (cardboard rack)
CT788-GS Bio Basic 1PK, 25UNIT

50ml Conical Centrifuge Tubes, Yellow Cap, Sterile, 25unit (cardboard rack)
CT788-YS Bio Basic 1PK, 25UNIT

MICRO CENTRIFUGE TUBE, AMBER, 5 ML, 250/1250
MCT-500-X CORNING 250/pk

DB 3.1 Single block centrifuge tube combination 4 x 1.5; 3 x 15 ml and 2 x 50ml - EACH
BLO6036 Scientific Laboratory Supplies EACH

50 mL Centrifuge Tube, Self-Standing, Bulk, Sterile, 25/pk, 500/cs
602072 NEST Biotechnology 500 pcs/cs

Corning 15mL PP Centrifuge Tubes Rack Packed with Plug Seal Cap Sterile 50/Rack 500/Case - PK500
430052 Scientific Laboratory Supplies PK500

Corning 15mL PET Centrifuge Tubes Rack Packed with Plug Seal Cap Sterile 50/Rack 500/Case - PK500
430055 Scientific Laboratory Supplies PK500

MICRO CENTRIFUGE TUBE, MAXYCLEAR, 5 ML, 250/1250
MCT-500-C CORNING 250/pk

Centrifuge Tube
C1005-T5*-Clear Benchmark Scientific 1 PC

50ml PP centrifuge tube, free standing, flat screw cap, 25/sterile bag, 500/cs
C2605 MTC Bio 500/pack

30ml PP centrifuge tube, free standing, flat screw cap, 25/sterile bag, 500/cs
C2630 MTC Bio 500/pack

Rack for 4 x 500mL centrifuge tubes, plastic coated wire, autoclavable, 1/ea
C1500-RK MTC Bio 1

50 mL Centrifuge Tube, Amber Color, Rack (PS Rack), Sterile, 25/pk, 500/cs
602201 NEST Biotechnology 500 pcs/cs

Sterile Centrifuge Tube 5mL (10 bags of 20) - PK200
CEN2053 Scientific Laboratory Supplies PK200

Sterile Centrifuge Tube, 5ml, 200 per. pk. (10 bags of 20)
C1005-T5-ST Benchmark Scientific 1 PC

Corning 500mL PP Centrifuge Tubes with Plug Seal Cap Sterile 6/Pack 36/Case - PK36
431123 Scientific Laboratory Supplies PK36

Kimax Reuseable Glass Centrifuge tubes graduated beaded top white scale 15ml - PK12
TUB2710 Scientific Laboratory Supplies PK12

Kimax Reuseable Glass Centrifuge tubes graduated beaded top white scale 50ml - PK12
TUB2712 Scientific Laboratory Supplies PK12

Corning 250mL PP Centrifuge Tubes with Plug Seal Cap Sterile 6/Pack 102/Case - PK102
430776 Scientific Laboratory Supplies PK102

MICRO CENTRIFUGE TUBE, MAXYCLEAR, 5 ML, STERILE, 250/1250
MCT-500-C-S CORNING 250/pk

FALCON®POLYSTYRENE PLUG SEAL SCREW CAPS FOR 50ML CONICAL BOTTOM CENTRIFUGE TUBES, STERILE, 50/PACK, 1000/CASE
358206 CORNING 50/pk

Kimax Centrifuge tubes disposable borosilicate glass plain snap cap finish 15ml - PK125
TUB2706 Scientific Laboratory Supplies PK125

Corning 15mL PP Centrifuge Tubes Bulk Packed with CentriStar Cap Sterile 25/Sleeve 500/Case - PK500
430791 Scientific Laboratory Supplies PK500

Kimax Centrifuge tubes disposable borosilicate glass plain screw thread finish 50ml - PK72
TUB2704 Scientific Laboratory Supplies PK72

Aluminum Insert Block with 24 positions for 15ml Centrifuge tubes (Use with small system)
109AYH-2416-15 Glascol each

Kimax Centrifuge tubes disposable borosilicate glass plain screw thread finish 10ml - PK125
TUB2700 Scientific Laboratory Supplies PK125

Kimax Centrifuge tubes disposable borosilicate glass plain screw thread finish 15ml - PK125
TUB2702 Scientific Laboratory Supplies PK125

Oil Centrifuge Tube 100ml - PK2
F87990 Scientific Laboratory Supplies PK2

FALCON® 15ML HIGH CLARITY PP CENTRIFUGE TUBE, CONICAL BOTTOM, WITH DOME-SEAL SCREW CAP, STERILE, 50/BAG, 500/CASE
352196 CORNING 50/pk

Corning 500ml Centrifuge tube with MLL/FLL dip tube and filter - PK2
11750 Scientific Laboratory Supplies PK2

Eppendorf Fixed-angle rotor F-35-30-17 for Centrifuge 5702 30 x 15ml Falcon Tubes - EACH
E5702704008 Scientific Laboratory Supplies EACH

Corning 50mL PP Centrifuge Tubes Conical Bottom CentriStar Cap Bulk Packed Sterile 25/Sleeve500/Case - PK500
430829 Scientific Laboratory Supplies PK500

Aluminum Insert Block with 12 positions for up to 50ml centrifuge tubes (Use with small system)
109AYH-2429-50 Glascol each

Aluminum Insert Block with 24 positions for up to 50ml centrifuge tubes (Use with large system)
109AYH-4829-50 Glascol each

Corning 50mL PP Centrifuge Tubes Conical Bottom CentriStar Cap Rack Packed Sterile 25/Rack 500/Case - PK500
430828 Scientific Laboratory Supplies PK500

Corning 15mL PP Centrifuge Tubes Bulk Packed with Plug Seal Cap Sterile 25/Sleeve 500/Case - PK500
430766 Scientific Laboratory Supplies PK500

Corning 50mL PP Centrifuge Tubes Self-Standing CentriStar Cap Bulk Packed Sterile 25/Sleeve 500/Case - PK500
430921 Scientific Laboratory Supplies PK500

Centrifuge Tube ASTM 100ml - PK2
362338 Scientific Laboratory Supplies PK2

11-Well Centrifuge Tube Box
R011-N Bio Basic 1 UNIT

12-Well Centrifuge Tube Box
R012-N Bio Basic 1 UNIT

24-Well Centrifuge Tube Box
R024-N Bio Basic 1 UNIT

Corning 15mL PET Centrifuge Tubes Bulk Packed with Plug Seal Cap Sterile 25/Sleeve 500/Case - PK500
430053 Scientific Laboratory Supplies PK500

Centrifuge 5920R 15/50mL package
E5948000460 Scientific Laboratory Supplies EACH

Centrifuge 5920R 15/50mL package
E5948000560 Scientific Laboratory Supplies EACH

Centrifuge Tube 15ML, Mauve cap
PW1306N-100X1NO EWC Diagnostics 1 unit

Centrifuge Tube 15ML, Mauve cap
PW1306N-20X25NO EWC Diagnostics 1 unit

Centrifuge Tube 15ML, Mauve cap
PW1306N-4X25NO EWC Diagnostics 1 unit

Centrifuge Tube 50ML, Mauve cap
PW1309N-100X1NO EWC Diagnostics 1 unit

Centrifuge Tube 50ML, Mauve cap
PW1309N-20X25NO EWC Diagnostics 1 unit

Centrifuge Tube 50ML, Mauve cap
PW1309N-4X25NO EWC Diagnostics 1 unit

Nunc Centrifuge Tube 11mm - PK480
337846 Scientific Laboratory Supplies PK480

Bottle Centrifuge Pp 250 Ml - PK4
3141-0250 Scientific Laboratory Supplies PK4

Bottle Centrifuge Pp 450 Ml - PK4
3141-0500 Scientific Laboratory Supplies PK4

DB 2.2 Single block for 5 x 50ml conical centrifuge tubes or 6; 12/13 or 25mm test tube combinations - EACH
BLO6034 Scientific Laboratory Supplies EACH

Sterile 50mL Centrifuge Tube (TPP)
TUBE50TPP-S Next Advance 320box

Centrifuge tube 1.5ml natural - PK500
CEN9308M Scientific Laboratory Supplies PK500

Micro Centrifuge Tube - B Attached
PW146-1X500NO EWC Diagnostics 1 unit

Micro Centrifuge Tube-C Attached
PW147-1X500NO EWC Diagnostics 1 unit

Micro Centrifuge Tube-D Attached
PW276-1X500NO EWC Diagnostics 1 unit

Centrifuge Tube Nalge 50Ml Pp - PK10
CEN9058 Scientific Laboratory Supplies PK10

Brand Blaubrand Centrifuge Tube - PK2
BR362038-2EA Scientific Laboratory Supplies PK2

Corning 50mL PP Centrifuge Tubes Conical Bottom Plug Seal Cap Rack Packed Sterile 25/Rack 500/Case - PK500
430290 Scientific Laboratory Supplies PK500

Corning 50mL PET Centrifuge Tubes Conical Bottom Plug Seal Cap Rack Packed Sterile 25/Rack 500/Case - PK500
430304 Scientific Laboratory Supplies PK500

Centrifuge Tube Oak Ridge 50Ml. - PK10
3139-0050 Scientific Laboratory Supplies PK10

CENTRIFUGE TUBE,50ML,NO CAP,S,25/500
431472 CORNING 25/pk

SLS Select Sterile Skirted Centrifuge Tube DNase and RNase free 50ml Sterile Bagged - PK500
SLS8114 Scientific Laboratory Supplies PK500
The highest encapsulation efficiency achieved was 36.9±3.8% and 79.8±6.4% for 5FU and Et (1:1), respectively. Growth inhibition studies in FaDu cells using different concentrations of 5FU and Et showed a combination index of 0.36, indicating a synergistic effect. The FaDu cell uptake of drug-loaded transfersomes was significantly (p<0.05) greater than that of free drugs. The transfersome hydrogel made of HPMC (2% w/w) showed similar flux, lag time, and permeation coefficient as that of drug-loaded transfersomes across excised porcine buccal tissue. In conclusion, 5FU and Et transfersome hydrogel can be developed for localized delivery to treat oral cancer.

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